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ELMO2 antibody [C3], C-term

Overexpression
Anti-ELMO2 antibody [C3], C-term used in Western Blot (WB). GTX108070
Anti-ELMO2 antibody [C3], C-term used in Western Blot (WB). GTX108070
Anti-ELMO2 antibody [C3], C-term used in Western Blot (WB). GTX108070
Anti-ELMO2 antibody [C3], C-term used in Western Blot (WB). GTX108070

Cat. No. GTX108070

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ELMO2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Mouse brain , ELMO2 transfected 293T cell , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ELMO2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950194

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

engulfment and cell motility 2 , CED-12 , CED12 , Ced-12A , ELMO-2 , VMPI

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein. Similarity to a C. elegans protein suggests that this protein may function in phagocytosis of apoptotic cells and in cell migration. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ELMO2 antibody [C3], C-term used in Western Blot (WB). GTX108070

GTX108070 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELMO2 antibody [C3], C-term (GTX108070) diluted at 1:1000.

Anti-ELMO2 antibody [C3], C-term used in Western Blot (WB). GTX108070

GTX108070 WB Image

Overexpression

Western Blot analysis of ELMO2 expression in transfected 293T cell line by ELMO2 polyclonal antibody.
A: Non-transfected lysate.
B: ELMO2 transfected lysate.
12% SDS PAGE
GTX108070 diluted at 1:500

Anti-ELMO2 antibody [C3], C-term used in Western Blot (WB). GTX108070

GTX108070 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX108070 diluted at 1:1000

Anti-ELMO2 antibody [C3], C-term used in Western Blot (WB). GTX108070

GTX108070 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELMO2 antibody [C3], C-term (GTX108070) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169