GeneTex
United States (US)

ELMO2 antibody [C3], C-term

Overexpression
GTX108070_WB_19011507_118.jpg
GTX108070_39735_20160811_WB_R_19011507_697.jpg
GTX108070_39735_WB_M_brain_19011507_855.jpg
GTX108070_39735_20160825_WB_19011507_726.jpg

Cat No. GTX108070

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , ELMO2 transfected 293T cell line , Mouse brain , PC-12 , Rat2

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ELMO2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Engulfment And Cell Motility 2,Ced-12,Ced12,Ced-12A,Elmo-2,Vmpi,Elmo2

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein. Similarity to a C. elegans protein suggests that this protein may function in phagocytosis of apoptotic cells and in cell migration. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108070_WB_19011507_118.jpg

GTX108070 WB Image

Overexpression

Western Blot analysis of ELMO2 expression in transfected 293T cell line by ELMO2 polyclonal antibody.
A: Non-transfected lysate.
B: ELMO2 transfected lysate.
12% SDS PAGE
GTX108070 diluted at 1:500

GTX108070_39735_20160811_WB_R_19011507_697.jpg

GTX108070 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELMO2 antibody [C3], C-term (GTX108070) diluted at 1:1000.

GTX108070_39735_WB_M_brain_19011507_855.jpg

GTX108070 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX108070 diluted at 1:1000

GTX108070_39735_20160825_WB_19011507_726.jpg

GTX108070 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELMO2 antibody [C3], C-term (GTX108070) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169