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ELMO3 antibody, C-term

Anti-ELMO3 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89943
Anti-ELMO3 antibody, C-term used in Western Blot (WB). GTX89943

Cat. No. GTX89943

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all ELMO3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 5-10μg/ml

Note :

IHC-P
Human Prostate shows vesiculate staining in the cytoplasm of secretory cells.
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89943-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence TNFNFCYDCSIAEP, from the C Terminus of the protein sequence according to NP_078988.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

engulfment and cell motility 3 , CED-12 , CED12 , ELMO-3

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is similar to a C. elegans protein that functions in phagocytosis of apoptotic cells and in cell migration. Other members of this small family of engulfment and cell motility (ELMO) proteins have been shown to interact with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ELMO3 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89943

GTX89943 IHC-P Image

IHC-P analysis of human prostate using GTX89943 ELMO3 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-ELMO3 antibody, C-term used in Western Blot (WB). GTX89943

GTX89943 WB Image

WB analysis of human brain (Hipoocampus) lysate using GTX89943 ELMO3 antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399