APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa nuclear extract , 293T , A431 , HeLa , HepG2
Predict Reactivity
Mouse, Zebrafish, Yeast, Drosophila, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.35 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ELP3. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888245
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
elongator acetyltransferase complex subunit 3 , KAT9
Cellular Localization
Cytoplasm , Nucleus
Background
ELP3 is the catalytic subunit of the histone acetyltransferase elongator complex, which contributes to transcript elongation and also regulates the maturation of projection neurons (Creppe et al., 2009 [PubMed 19185337]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-ELP3 antibody [GT11811] used in Western Blot (WB). GTX631395](/templates/default/js/lightbox/images/loading.gif "Anti-ELP3 antibody [GT11811] used in Western Blot (WB). GTX631395")
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GTX631395 WB Image
ELP3 antibody detects ELP3 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody (GTX631395) diluted by 1:1000.
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GTX631395 IP Image
Immunoprecipitation of ELP3 protein from 293T whole cell extracts using 5 μg of ELP3 antibody [GT11811] (GTX631395).
Western blot analysis was performed using ELP3 antibody [GT11811] (GTX631395).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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GTX631395 WB Image
ELP3 antibody detects ELP3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody (GTX631395) diluted by 1:500.
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GTX631395 ICC/IF Image
ELP3 antibody [GT11811] detects ELP3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ELP3 protein stained by ELP3 antibody [GT11811] (GTX631395) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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REFERENCE
There are currently no references for ELP3 antibody [GT11811] (GTX631395). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ELP3 antibody [GT11811] (GTX631395). Be the first to share your experience with this product.
![Anti-ELP3 antibody [GT11811] used in Western Blot (WB). GTX631395](/upload/website/prouct_img/small/GTX631395/GTX631395_41750_WB_Fraction_w_23061202_975.webp "Anti-ELP3 antibody [GT11811] used in Western Blot (WB). GTX631395")
![Anti-ELP3 antibody [GT11811] used in Immunoprecipitation (IP). GTX631395](/upload/website/prouct_img/small/GTX631395/GTX631395_41750_20150618_IP_w_23061202_168.webp "Anti-ELP3 antibody [GT11811] used in Immunoprecipitation (IP). GTX631395")
![Anti-ELP3 antibody [GT11811] used in Western Blot (WB). GTX631395](/upload/website/prouct_img/small/GTX631395/GTX631395_41750_WB_w_23061202_648.webp "Anti-ELP3 antibody [GT11811] used in Western Blot (WB). GTX631395")
![Anti-ELP3 antibody [GT11811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631395](/upload/website/prouct_img/small/GTX631395/GTX631395_41750_IFA_w_23061202_533.webp "Anti-ELP3 antibody [GT11811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631395")
![Anti-ELP3 antibody [GT11811] used in Western Blot (WB). GTX631395](/upload/website/prouct_img/normal/GTX631395/GTX631395_41750_WB_Fraction_w_23061202_975.webp "Anti-ELP3 antibody [GT11811] used in Western Blot (WB). GTX631395")
![Anti-ELP3 antibody [GT11811] used in Immunoprecipitation (IP). GTX631395](/upload/website/prouct_img/normal/GTX631395/GTX631395_41750_20150618_IP_w_23061202_168.webp "Anti-ELP3 antibody [GT11811] used in Immunoprecipitation (IP). GTX631395")
![Anti-ELP3 antibody [GT11811] used in Western Blot (WB). GTX631395](/upload/website/prouct_img/normal/GTX631395/GTX631395_41750_WB_w_23061202_648.webp "Anti-ELP3 antibody [GT11811] used in Western Blot (WB). GTX631395")
![Anti-ELP3 antibody [GT11811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631395](/upload/website/prouct_img/normal/GTX631395/GTX631395_41750_IFA_w_23061202_533.webp "Anti-ELP3 antibody [GT11811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631395")