ELP4 antibody

Cat. No. GTX111748

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all ELP4 products

Datasheet
Document

View product citations for antibody GTX111748 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ELP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618449

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

elongator acetyltransferase complex subunit 4 , AN , AN2 , C11orf19 , PAX6NEB , PAXNEB , dJ68P15A.1 , hELP4

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a component of the six subunit elongator complex, a histone acetyltransferase complex that associates directly with RNA polymerase II during transcriptional elongation. The human gene can partially complement sensitivity phenotypes of yeast ELP4 deletion mutants. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq]

Database

Gene ID: 26610 ELP4
UniProt: Q96EB1 ELP4

Research Area

DATA IMAGES

GTX111748 IP Image

ELP4 antibody immunoprecipitates ELP4 protein in IP experiments.
IP Sample: HeLa whole cell lysate/extract
A. Control with 2 μg of preimmune rabbit IgG
B. Immunoprecipitation of ELP4 protein by 2 μg of ELP4 antibody (GTX111748)
10% SDS-PAGE
The immunoprecipitated ELP4 protein was detected by ELP4 antibody (GTX111748) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX111748 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX111748 diluted at 1:1000

GTX111748 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using ELP4(GTX111748) antibody at 1:500 dilution.

GTX111748 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using ELP4(GTX111748) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty