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EMAP II antibody

Anti-EMAP II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02201
Anti-EMAP II antibody used in IHC (Paraffin sections) (IHC-P). GTX02201
Anti-EMAP II antibody used in Western Blot (WB). GTX02201
Anti-EMAP II antibody used in IHC (Paraffin sections) (IHC-P). GTX02201
Anti-EMAP II antibody used in Immunoprecipitation (IP). GTX02201

Cat No. GTX02201

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:200-1:1000
IHC-P 1:1000-1:10000
IP 1-5 μl/mg of lysate
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human EMAP-II / AIMP1 protein

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aminoacyl tRNA synthetase complex interacting multifunctional protein 1 , EMAP2 , EMAPII , HLD3 , SCYE1 , p43

Cellular Localization

Nucleus , Cytoplasm, cytosol , Secreted , Endoplasmic reticulum , Golgi apparatus

Background

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EMAP II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02201

GTX02201 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02201 EMAP II antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500
Permeabilzation : 0.1% Triton X-100

Anti-EMAP II antibody used in IHC (Paraffin sections) (IHC-P). GTX02201

GTX02201 IHC-P Image

IHC-P analysis of human pancreas tissue section using GTX02201 EMAP II antibody.
Dilution : 1:5000

Anti-EMAP II antibody used in Western Blot (WB). GTX02201

GTX02201 WB Image

WB analysis of various samples using GTX02201 EMAP II antibody.
Lane A : H1299 whole cell lysate
Lane B : HepG2 whole cell lysate
Lane C : HEK293 whole cell lysate
Lane D : Jurkat whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-EMAP II antibody used in IHC (Paraffin sections) (IHC-P). GTX02201

GTX02201 IHC-P Image

IHC-P analysis of human brain tissue section using GTX02201 EMAP II antibody.
Dilution : 1:5000

Anti-EMAP II antibody used in Immunoprecipitation (IP). GTX02201

GTX02201 IP Image

IP analysis of HepG2 whole cell lysate using GTX02201 EMAP II antibody.
IP antibody : 4 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
Immunomagnetic beads Protein A/G : 60 μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 339