GeneTex
United States (US)

EMAP II antibody

GTX12442_2_ELISA_20180221_18121410_714.jpg
GTX12442_2284_WB_20180221_18121410_604.jpg
GTX12442_2283_WB_20180221_18121410_308.jpg

Cat No. GTX12442

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, sELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 - 0.2 μg/mL
ELISA 0.5 μg/mL
sELISA 0.5 - 2.0 ug/ml

Note :

sELISA
Capture : GTX12442, Detection : Biotinylated Anti-Human EMAP-II antibody

Not tested in other applications.

Calculated MW

34 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.5X PBS. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEMAP-II (human EMAP-II).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aminoacyl Trna Synthetase Complex Interacting Multifunctional Protein 1,Emap2,Emapii,Hld3,Scye1,P43,Aimp1

Cellular Localization

Secreted

Background

EMAP II is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor associated vasculature sensitive to tumor necrosis factor. Its precursor is identical to the p43 subunit, which is associated with the multi tRNA synthetase complex. Therefore, proEMAP II may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.

Database

Research Area

DATA IMAGES
GTX12442_2_ELISA_20180221_18121410_714.jpg

GTX12442 ELISA Image

ELISA analysis of human EMAP-II recombinant protein(0.2 - 0.4 ng/well) using GTX12442 EMAP II antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Human EMAP-II as a detection antibody.

GTX12442_2284_WB_20180221_18121410_604.jpg

GTX12442 WB Image

WB analysis of sequential diluted human EMAP-II recombinant protein under either reducing or non-reducing conditions using GTX12442 EMAP II antibody.
Working concentration : 0.1 - 0.2 ug/ml

GTX12442_2283_WB_20180221_18121410_308.jpg

GTX12442 WB Image

WB analysis of sequential diluted human EMAP-II recombinant protein under either reducing or non-reducing conditions using GTX12442 EMAP II antibody.
Working concentration : 0.1 - 0.2 ug/ml

Package List Price ($)
$ 319