APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1 - 0.2 μg/mL |
| 0.5 μg/mL |
| 0.5 - 2.0 ug/ml |
|
Note :
Sandwich ELISA
Capture : GTX12442, Detection : Biotinylated Anti-Human EMAP-II antibody
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
0.5x PBS pH7.4
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEMAP-II (human EMAP-II).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aminoacyl tRNA synthetase complex interacting multifunctional protein 1 , EMAP2 , EMAPII , HLD3 , SCYE1 , p43
Cellular Localization
Nucleus,Cytoplasm, cytosol,Secreted,Endoplasmic reticulum,Golgi apparatus
Background
EMAP II is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor associated vasculature sensitive to tumor necrosis factor. Its precursor is identical to the p43 subunit, which is associated with the multi tRNA synthetase complex. Therefore, proEMAP II may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.
Database
Research Area
DATA IMAGES
|
GTX12442 ELISA Image
ELISA analysis of human EMAP-II recombinant protein(0.2 - 0.4 ng/well) using GTX12442 EMAP II antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Human EMAP-II as a detection antibody.
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|
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GTX12442 WB Image
WB analysis of sequential diluted human EMAP-II recombinant protein under either reducing or non-reducing conditions using GTX12442 EMAP II antibody.
Working concentration : 0.1 - 0.2 ug/ml
|
|
|
GTX12442 WB Image
WB analysis of sequential diluted human EMAP-II recombinant protein under either reducing or non-reducing conditions using GTX12442 EMAP II antibody.
Working concentration : 0.1 - 0.2 ug/ml
|
REFERENCE
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REVIEW
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