Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EMB antibody [N3C3] (GTX121952) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A431, using EMB(GTX121952) antibody at 1:500 dilution.