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EMI1 antibody [EMI1/1176]

Anti-EMI1 antibody [EMI1/1176] used in IHC (Paraffin sections) (IHC-P). GTX34689
Anti-EMI1 antibody [EMI1/1176] used in Western Blot (WB). GTX34689
Anti-EMI1 antibody [EMI1/1176] used in IHC (Paraffin sections) (IHC-P). GTX34689

Cat No. GTX34689

Host Mouse
Clonality Monoclonal
Clone Name EMI1/1176
Isotype IgG2a
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μg ($429)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2ug/ml for 60 minutes at RT
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
PROPERTIES

Form

Liquid

Buffer

1mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

F-Box Protein 5 , Emi1 , Fbx5 , Fbxo31 , Fbxo5

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES
Anti-EMI1 antibody [EMI1/1176] used in IHC (Paraffin sections) (IHC-P). GTX34689

GTX34689 IHC-P Image

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).

Anti-EMI1 antibody [EMI1/1176] used in Western Blot (WB). GTX34689

GTX34689 WB Image

Western Blot of HeLa, HepG2, HEK293 and K562 Cell Lysates using EMI1 Monoclonal Antibody (EMI1/1176).

Anti-EMI1 antibody [EMI1/1176] used in IHC (Paraffin sections) (IHC-P). GTX34689

GTX34689 IHC-P Image

Formalin-fixed, paraffin-embedded human Ovarian carcinoma stained with EMI1 Monoclonal Antibody (EMI1/1176).

REFERENCE
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REVIEW
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Package List Price ($)
$ 429