GeneTex
United States (US)

EML1 antibody [C3], C-term

GTX100252_WB_19011506_365.jpg
GTX100252_39421_IHC_M_19011506_973.jpg
GTX100252_WB_M_19011506_715.jpg
GTX100252_39421_IHC_19011506_856.jpg
GTX100252_39421_20150410_IFA_19011506_612.jpg
GTX100252_39421_IHC_R_19011506_540.jpg

Cat No. GTX100252

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

H1299 , NIH-3T3

Predict Reactivity

Zebrafish(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EML1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ELP79 antibody, EMAP antibody, EMAPL antibody, FLJ45033 antibody, HuEMAP antibody, EML1 antibody, huEMAP-1 antibody, echinoderm microtubule-associated protein-like 1 antibody, EMAP-1 antibody, echinoderm microtubule associated protein like 1 antibody

Cellular Localization

Cytoplasm

Background

Human echinoderm microtubule-associated protein-like is a strong candidate for the Usher syndrome type 1A gene. Usher syndromes (USHs) are a group of genetic disorders consisting of congenital deafness, retinitis pigmentosa, and vestibular dysfunction of variable onset and severity depending on the genetic type. The disease process in USHs involves the entire brain and is not limited to the posterior fossa or auditory and visual systems. The USHs are catagorized as type I (USH1A, USH1B, USH1C, USH1D, USH1E and USH1F), type II (USH2A and USH2B) and type III (USH3). The type I is the most severe form. Gene loci responsible for these three types are all mapped. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX100252_WB_19011506_365.jpg

GTX100252 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
EML1 antibody
GTX100252 diluted at 1:500

GTX100252_39421_IHC_M_19011506_973.jpg

GTX100252 IHC-P Image

EML1 antibody [C3], C-term detects EML1 protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
EML1 antibody [C3], C-term (GTX100252) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100252_WB_M_19011506_715.jpg

GTX100252 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX100252 diluted at 1:1000

GTX100252_39421_IHC_19011506_856.jpg

GTX100252 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RF48 xenograft, using EML1(GTX100252) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100252_39421_20150410_IFA_19011506_612.jpg

GTX100252 ICC/IF Image

EML1 antibody [c3], c-term detects EML1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EML1 protein stained by EML1 antibody [c3], c-term (GTX100252) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX100252_39421_IHC_R_19011506_540.jpg

GTX100252 IHC-P Image

EML1 antibody [C3], C-term detects EML1 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
EML1 antibody [C3], C-term (GTX100252) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169