EML2 antibody [N2C2], Internal

Cat. No. GTX115762

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all EML2 products

Datasheet
Document

View product citations for antibody GTX115762 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EML2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621969

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EMAP like 2 , ELP70 , EMAP-2 , EMAP2

Cellular Localization

Cytoplasm

Background

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.

Database

Gene ID: 24139 EML2
UniProt: O95834 EML2

Research Area

DATA IMAGES

GTX115762 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using EML2(GTX115762) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115762 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX115762 diluted at 1:500

GTX115762 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using EML2(GTX115762) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty