GeneTex
United States (US)

EMR2 antibody [2A1]

GTX31227_3599_FACS_18121410_786.jpg

Cat No. GTX31227

Host Mouse
Clonality Monoclonal
Clone Name 2A1
Isotype IgG1
Application WB, IHC-Fr, FACS, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-Fr 1/50-1/200
FACS 1/50-1/200
IP Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Specificity/Sensitivity

Clone 2A1 specifically recognizes the stalk region of EMR2.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adhesion G Protein-Coupled Receptor E2,Cd312,Emr2,Vbu,Adgre2

Background

This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES
GTX31227_3599_FACS_18121410_786.jpg

GTX31227 FACS Image

FACS analysis of human peripheral blood granulocytes using GTX31227 EMR2 antibody [2A1].

Package List Price ($)
$ 319