Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1/50-1/200 |
1/50-1/200 |
Assay dependent |
Note :
FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
|
Not tested in other applications.
Calculated MW
Product Note
Clone 2A1 specifically recognizes the stalk region of EMR2.
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA
Purification
Protein A purified
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
adhesion G protein-coupled receptor E2 , CD312 , CD97 , EMR2 , VBU
Cellular Localization
Cell membrane
Background
This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
Database
Research Area