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EN1 antibody, N-term

Anti-EN1 antibody, N-term used in Western Blot (WB). GTX81554
Anti-EN1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81554
Anti-EN1 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81554
Anti-EN1 antibody, N-term used in Western Blot (WB). GTX81554
Anti-EN1 antibody, N-term used in Western Blot (WB). GTX81554
Anti-EN1 antibody, N-term used in Western Blot (WB). GTX81554
Anti-EN1 antibody, N-term used in Western Blot (WB). GTX81554

Cat. No. GTX81554

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
200 μl ($399)
See all EN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

engrailed homeobox 1

Cellular Localization

Nucleus

Background

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EN1 antibody, N-term used in Western Blot (WB). GTX81554

GTX81554 WB Image

WB analysis of various samples using GTX81554 EN1 antibody, N-term.
Lane 1: U-2OS whole cell lysate
Lane 2: U87-MG whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Lane 4: 293T/17 whole cell lysate
Lane 5: PC-3 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:2000

Anti-EN1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81554

GTX81554 IHC-P Image

IHC-P analysis of human tonsil using GTX81554 EN1 antibody, N-term.
Antigen retrieval : heat-induced antigen retrieval with citrate buffer (pH6)
Dilution : 1:25

Anti-EN1 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81554

GTX81554 ICC/IF Image

ICC/IF analysis of U-2 OS cells using GTX81554 EN1 antibody, N-term.
Green : EN1
Red : Actin filament
Fixation : 4% paraformaldehyde (PFA)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25

Anti-EN1 antibody, N-term used in Western Blot (WB). GTX81554

GTX81554 WB Image

WB analysis of various samples using GTX81554 EN1 antibody, N-term. Lane 1: NCI-H460 whole cell lysates Lane 2: SH-SY5Y whole cell lysates Lane 3: PC-3 whole cell lysates Lane 4: Jurkat whole cell lysates Lane 5: 293T whole cell lysates
Loading : 20 μg per lane
Dilution : 1:2000

Anti-EN1 antibody, N-term used in Western Blot (WB). GTX81554

GTX81554 WB Image

WB analysis of 293, SH-SY5Y, NCI-H460 cell using GTX81554 EN1 antibody, N-term.
Loading : 20ug per lane
Dilution : 1:1000

Anti-EN1 antibody, N-term used in Western Blot (WB). GTX81554

GTX81554 WB Image

WB analysis of various samples using GTX81554 EN1 antibody, N-term.
Lane 1: human fetal brain lysate
Lane 2: SH-SY5Y whole cell lysate
Loading : 20 μg per lane
Dilution : 1:1000

Anti-EN1 antibody, N-term used in Western Blot (WB). GTX81554

GTX81554 WB Image

WB analysis of various samples using GTX81554 EN1 antibody, N-term.
Lane 1: U-2OS whole cell lysate
Lane 2: HepG2 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399