WB analysis of various samples using GTX81554 EN1 antibody, N-term. Lane 1: NCI-H460 whole cell lysates Lane 2: SH-SY5Y whole cell lysates Lane 3: PC-3 whole cell lysates Lane 4: Jurkat whole cell lysates Lane 5: 293T whole cell lysates
Loading : 20 μg per lane
Dilution : 1:2000

ICC/IF analysis of U-2 OS cells using GTX81554 EN1 antibody, N-term.
Green : EN1
Red : Actin filament
Fixation : 4% paraformaldehyde (PFA)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25

IHC-P analysis of human tonsil using GTX81554 EN1 antibody, N-term.
Antigen retrieval : heat-induced antigen retrieval with citrate buffer (pH6)
Dilution : 1:25

WB analysis of various samples using GTX81554 EN1 antibody, N-term.
Lane 1: U-2OS whole cell lysate
Lane 2: U87-MG whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Lane 4: 293T/17 whole cell lysate
Lane 5: PC-3 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:2000

WB analysis of various samples using GTX81554 EN1 antibody, N-term.
Lane 1: U-2OS whole cell lysate
Lane 2: HepG2 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:1000

WB analysis of various samples using GTX81554 EN1 antibody, N-term.
Lane 1: human fetal brain lysate
Lane 2: SH-SY5Y whole cell lysate
Loading : 20 μg per lane
Dilution : 1:1000

WB analysis of 293, SH-SY5Y, NCI-H460 cell using GTX81554 EN1 antibody, N-term.
Loading : 20ug per lane
Dilution : 1:1000