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EN1 antibody

Anti-EN1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134013
Anti-EN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134013
Anti-EN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134013

Cat. No. GTX134013

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, IHC-Fr

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all EN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse EN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887172

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

engrailed 1 , En-1 , Mo-en.1 , engrailed-1

Background

RNA-binding protein. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.

Database

Research Area

DATA IMAGES

Anti-EN1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX134013

GTX134013 IHC-Fr Image

EN1 antibody detects EN1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: EN1 stained by EN1 antibody (GTX134013) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-EN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134013

GTX134013 IHC-P Image

EN1 antibody detects EN1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
EN1 stained by EN1 antibody (GTX134013) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134013

GTX134013 IHC-P Image

EN1 antibody detects EN1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
EN1 stained by EN1 antibody (GTX134013) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169