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EN2 antibody, C-term

Anti-EN2 antibody, C-term used in Western Blot (WB). GTX47599

Cat No. GTX47599

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX47599-PEP).

Predict Reactivity

Human, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Engrailed Homeobox 2 , En2

Background

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EN2 antibody, C-term used in Western Blot (WB). GTX47599

GTX47599 WB Image

WB analysis of mouse brain lysate using GTX47599 EN2 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf