EN2 antibody immunoprecipitates EN2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of EN2 protein by 2 μg of EN2 antibody (GTX109198) 12% SDS-PAGE The immunoprecipitated EN2 protein was detected by EN2 antibody (GTX109198) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 ug of whole cell lysate)
A: MCF-7
12% SDS PAGE
GTX109198 diluted at 1:10000