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ENO1 antibody [GT186]

Anti-ENO1 antibody [GT186] used in Western Blot (WB). GTX630506
Anti-ENO1 antibody [GT186] used in Western Blot (WB). GTX630506

Cat No. GTX630506

Host Mouse
Clonality Monoclonal
Clone Name GT186
Isotype IgG2a
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A549 , H1299 , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Guinea pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

enolase 1,ENO1L1,HEL-S-17,MPB1,NNE,PPH

Cellular Localization

Cytoplasm , Cell membrane , myofibril , sarcomere , M-band , Isoform MBP-1: Nucleus

Background

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ENO1 antibody [GT186] used in Western Blot (WB). GTX630506

GTX630506 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] (GTX630506) diluted at 1:15000.

Anti-ENO1 antibody [GT186] used in Western Blot (WB). GTX630506

GTX630506 WB Image

ENO1 antibody [GT186] detects ENO1 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
ENO1 antibody [GT186] (GTX630506) dilution: 1:10000

REFERENCE
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REVIEW

ENO1 antibody [GT186] Cat No. GTX630506

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-Oct-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1299 cells
Amount used : 30μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 20Hr
SDS
PBS.pdf