Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] (GTX630506) diluted at 1:15000.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] (GTX630506) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

ENO1 antibody [GT186] detects ENO1 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
ENO1 antibody [GT186] (GTX630506) dilution: 1:10000

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] (GTX630506) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.