ENO1 antibody [GT186]

Name: ENO1 antibody [GT186]
Brand: GeneTex
SKU: GTX630506
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-ENO1 antibody [GT186] used in Western Blot (WB). GTX630506

Cat. No. GTX630506

Host	Mouse
Clonality	Monoclonal
Clone Name	GT186
Isotype	IgG2a
Application	WB
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

See all ENO1 products

Datasheet
Document

View product citations for antibody GTX630506 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , 293T

Predict Reactivity

Mouse, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888212

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enolase 1 , ENO1L1 , HEL-S-17 , MPB1 , NNE , PPH

Cellular Localization

Cytoplasm , Cell membrane , myofibril , sarcomere , M-band , Isoform MBP-1: Nucleus

Background

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

Database

Gene ID: 2023 ENO1
UniProt: P06733 ENO1

Research Area

DATA IMAGES

GTX630506 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] (GTX630506) diluted at 1:15000.

GTX630506 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] (GTX630506) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX630506 WB Image

ENO1 antibody [GT186] detects ENO1 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
10 % SDS-PAGE
ENO1 antibody [GT186] (GTX630506) dilution: 1:10000

GTX630506 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] (GTX630506) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW