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ENO1 antibody, Internal

Anti-ENO1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX49060
Anti-ENO1 antibody, Internal used in Western Blot (WB). GTX49060

Cat No. GTX49060

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Jurkat

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human Enolase 1

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enolase 1 , ENO1L1 , HEL-S-17 , MPB1 , NNE , PPH

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-ENO1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX49060

GTX49060 IHC-P Image

IHC-P analysis of human liver tissue using GTX49060 Enolase 1 antibody at 5μg/ml.

Anti-ENO1 antibody, Internal used in Western Blot (WB). GTX49060

GTX49060 WB Image

WB analysis of Jurkat cells using GTX49060 Enolase 1 antibody at 0.5μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf