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ENO1 antibody [JM63-53]

Recombinant antibody
Anti-ENO1 antibody [JM63-53] used in Western Blot (WB). GTX00990
Anti-ENO1 antibody [JM63-53] used in Flow cytometry (FACS). GTX00990

Cat No. GTX00990

Host

Rabbit

Clonality

Monoclonal

Clone Name

JM63-53

Isotype

IgG

Application

WB, FACS

Reactivity

Human
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enolase 1,ENO1,ENO1L1,HELS17,MPB1,NNE,PPH,Non-Neuronal Enolase,Enolase 1

Cellular Localization

Cytoplasm,Cell membrane,Myofibril,Sarcomere,M line,Nucleus

Background

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-ENO1 antibody [JM63-53] used in Western Blot (WB). GTX00990

GTX00990 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [JM63-53] (GTX00990) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ENO1 antibody [JM63-53] used in Flow cytometry (FACS). GTX00990

GTX00990 FACS Image

FACS analysis of MCF-7 cells using GTX00990 ENO1 antibody [JM63-53].
Red : primary antibody
Black : unlabelled control
Dilution : 1:100

REFERENCE

There are currently no references for ENO1 antibody [JM63-53] (GTX00990). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ENO1 antibody [JM63-53] (GTX00990). Be the first to share your experience with this product.
Package List Price ($)
$ 369