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ENO1 antibody [N3C3]

KO/KD
Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803
Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803
Anti-ENO1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101803
Anti-ENO1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101803
Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803
Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803
Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803
Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803

Cat No. GTX101803

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, Dot

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all ENO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Dot Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Bovine, Chicken, Guinea pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enolase 1 , ENO1L1 , HEL-S-17 , MPB1 , NNE , PPH

Cellular Localization

Cytoplasm , Cell membrane , myofibril , sarcomere , M-band , Isoform MBP-1: Nucleus

Background

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803

GTX101803 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803

GTX101803 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101803 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ENO1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101803

GTX101803 ICC/IF Image

ENO1 antibody [N3C3] detects ENO1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ENO1 protein stained by ENO1 antibody [N3C3] (GTX101803) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-ENO1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101803

GTX101803 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric tissue, using ENO1(GTX101803) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803

GTX101803 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
GTX101803 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803

GTX101803 WB Image

The WB analysis of ENO1 antibody [N3C3] was published by Lin LL and colleagues in the journal BMC Genomics in 2015.PMID: 25652794

Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803

GTX101803 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ENO1 antibody [N3C3] used in Western Blot (WB). GTX101803

GTX101803 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Zhou X et al. Technol Cancer Res Treat 2016; 15 (5) : 697-706 Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics.
Application : WB
Reactivity : Human
Lin LL et al. BMC Genomics 2015; 16 : 41 Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.
Application : WB
Reactivity : Human
Wu YH et al. PLoS One 2013; 8 (8) : e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.
Application : WB
Reactivity : Human
Hsiao KC et al. PLoS One 2013; 8 (7) : e69354 Surface α-enolase promotes extracellular matrix degradation and tumor metastasis and represents a new therapeutic target.
Application : Dot
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169