APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:500-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T
Predict Reactivity
Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.26 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10731276
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
enolase 1 , ENO1L1 , HEL-S-17 , MPB1 , NNE , PPH
Cellular Localization
Cytoplasm , Cell membrane , myofibril , sarcomere , M-band , Isoform MBP-1: Nucleus
Background
This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113179 IP Image
ENO1antibody immunoprecipitates ENO1protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of ENO1protein by 2.5 μg of ENO1 antibody (GTX113179) 10% SDS-PAGE The immunoprecipitated ENO1 protein was detected by ENO1 antibody (GTX113179) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX113179 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:20000.
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GTX113179 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ENO1(GTX113179) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113179 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113179 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113179 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HCT116, using ENO1(GTX113179) antibody at 1:500 dilution.
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GTX113179 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113179 WB Image
The data was published in the journal Viruses in 2018. PMID: 29518929
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REFERENCE
REVIEW
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