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ENO1 antibody

KO/KD
Anti-ENO1 antibody used in Immunoprecipitation (IP). GTX55604
Anti-ENO1 antibody used in Western Blot (WB). GTX55604
Anti-ENO1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55604
Anti-ENO1 antibody used in Western Blot (WB). GTX55604
Anti-ENO1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55604
Anti-ENO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55604

Cat. No. GTX55604

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all ENO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enolase 1 , ENO1L1 , HEL-S-17 , MPB1 , NNE , PPH

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-ENO1 antibody used in Immunoprecipitation (IP). GTX55604

GTX55604 IP Image

IP analysis of HeLa cell lysate using GTX55604 ENO1 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-ENO1 antibody used in Western Blot (WB). GTX55604

GTX55604 WB Image

WB analysis of various sample lysates using GTX55604 ENO1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ENO1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55604

GTX55604 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX55604 ENO1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ENO1 antibody used in Western Blot (WB). GTX55604

GTX55604 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX55604 ENO1 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-ENO1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55604

GTX55604 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX55604 ENO1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ENO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55604

GTX55604 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX55604 ENO1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399