GeneTex
United States (US)

ENO3 antibody

GTX113429_WB_18121410_762.jpg
GTX113429_40128_IHC_18121410_289.jpg
GTX113429_40128_WB_M_Brain_18121410_679.jpg
GTX113429_40128_WB_R_18121410_869.jpg

Cat No. GTX113429

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji , mouse brain , Rat2

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENO3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GSD13 antibody, MSE antibody, ENO3 antibody, beta-enolase antibody, skeletal muscle enolase antibody, muscle-specific enolase antibody, 2-phospho-D-glycerate hydrolyase antibody, enolase 3 (beta, muscle) antibody

Cellular Localization

Cytoplasm

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX113429_WB_18121410_762.jpg

GTX113429 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
C: Raji
10% SDS PAGE
GTX113429 diluted at 1:1000

GTX113429_40128_IHC_18121410_289.jpg

GTX113429 IHC-P Image

ENO3 antibody detects ENO3 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
ENO3 antibody (GTX113429) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113429_40128_WB_M_Brain_18121410_679.jpg

GTX113429 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113429 diluted at 1:10000

GTX113429_40128_WB_R_18121410_869.jpg

GTX113429 WB Image

ENO3 antibody detects ENO3 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
ENO3 antibody (GTX113429) dilution: 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169