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ENO3 antibody

Anti-ENO3 antibody used in Western Blot (WB). GTX113429
Anti-ENO3 antibody used in IHC (Whole mount) (IHC-Wm). GTX113429
Anti-ENO3 antibody used in Western Blot (WB). GTX113429
Anti-ENO3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113429
Anti-ENO3 antibody used in Western Blot (WB). GTX113429
Anti-ENO3 antibody used in Western Blot (WB). GTX113429

Cat. No. GTX113429

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all ENO3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji , Mouse brain , Rat2

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENO3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036856

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enolase 3 , GSD13 , MSE

Cellular Localization

Cytoplasm

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ENO3 antibody used in Western Blot (WB). GTX113429

GTX113429 WB Image

Sample (30 μg of whole cell lysate)
A: Zebrafish muscle
10% SDS PAGE
GTX113429 diluted at 1:1000

Anti-ENO3 antibody used in IHC (Whole mount) (IHC-Wm). GTX113429

GTX113429 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using ENO3 antibody (GTX113429) at 1:200 dilution.

Anti-ENO3 antibody used in Western Blot (WB). GTX113429

GTX113429 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113429 diluted at 1:10000

Anti-ENO3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113429

GTX113429 IHC-P Image

ENO3 antibody detects ENO3 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
ENO3 antibody (GTX113429) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ENO3 antibody used in Western Blot (WB). GTX113429

GTX113429 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
C: Raji
10% SDS PAGE
GTX113429 diluted at 1:1000

Anti-ENO3 antibody used in Western Blot (WB). GTX113429

GTX113429 WB Image

ENO3 antibody detects ENO3 protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
ENO3 antibody (GTX113429) dilution: 1:1000

REFERENCE

Application Reference

Gueugneau M et al. BMC Genomics 2014; 15 (1) : 1165 Proteomics of muscle chronological ageing in post-menopausal women.
Application : WB
Reactivity : Human
Hadrevi J et al. BMC Musculoskelet Disord 2011; 12 : 181 Protein differences between human trapezius and vastus lateralis muscles determined with a proteomic approach.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169