GeneTex
United States (US)

ENSA antibody

KO/KD
GTX101493_40520_20180119_WB_KO_watermark_19011506_282.jpg
GTX101493_40250_IFA_19011506_675.jpg
GTX101493_40520_IHC_R_19011506_915.jpg
GTX101493_40520_IHC_M_19011506_800.jpg
GTX101493_40520_IHC_M_2_19011506_197.jpg

Cat No. GTX101493

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.6mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENSA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARPP-19e antibody, MGC4319 antibody, MGC78563 antibody, MGC8394 antibody, ENSA antibody, alpha-endosulfine antibody, endosulfine alpha antibody

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX101493_40520_20180119_WB_KO_watermark_19011506_282.jpg

GTX101493 WB Image

KO/KD

Wild-type (WT) and ENSA knockout (KO) 293T cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ENSA antibody (GTX101493) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101493_40250_IFA_19011506_675.jpg

GTX101493 ICC/IF Image

ENSA antibody detects ENSA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold Methanol for 5 min.
Green: ENSA protein stained by ENSA antibody (GTX101493) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX101493_40520_IHC_R_19011506_915.jpg

GTX101493 IHC-P Image

ENSA antibody detects ENSA protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
ENSA antibody (GTX101493) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101493_40520_IHC_M_19011506_800.jpg

GTX101493 IHC-P Image

ENSA antibody detects ENSA protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ENSA antibody (GTX101493) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101493_40520_IHC_M_2_19011506_197.jpg

GTX101493 IHC-P Image

ENSA antibody detects ENSA protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ENSA antibody (GTX101493) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf