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ENSA antibody

KO/KD
Anti-ENSA antibody used in Western Blot (WB). GTX101493
Anti-ENSA antibody used in IHC (Paraffin sections) (IHC-P). GTX101493
Anti-ENSA antibody used in IHC (Paraffin sections) (IHC-P). GTX101493
Anti-ENSA antibody used in IHC (Paraffin sections) (IHC-P). GTX101493
Anti-ENSA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101493

Cat. No. GTX101493

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ENSA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENSA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166482

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endosulfine alpha , ARPP-19e

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ENSA antibody used in Western Blot (WB). GTX101493

GTX101493 WB Image

KO/KD

Wild-type (WT) and ENSA knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ENSA antibody (GTX101493) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ENSA antibody used in IHC (Paraffin sections) (IHC-P). GTX101493

GTX101493 IHC-P Image

ENSA antibody detects ENSA protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ENSA antibody (GTX101493) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ENSA antibody used in IHC (Paraffin sections) (IHC-P). GTX101493

GTX101493 IHC-P Image

ENSA antibody detects ENSA protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ENSA antibody (GTX101493) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ENSA antibody used in IHC (Paraffin sections) (IHC-P). GTX101493

GTX101493 IHC-P Image

ENSA antibody detects ENSA protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
ENSA antibody (GTX101493) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ENSA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101493

GTX101493 ICC/IF Image

ENSA antibody detects ENSA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold Methanol for 5 min.
Green: ENSA protein stained by ENSA antibody (GTX101493) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

Application Reference

S Kim et al. BMC Cancer 2023; 23 (1) : 1263 Endosulfine alpha maintains spindle pole integrity by recruiting Aurora A during mitosis
Application : WB, ICC/IF
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169