APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Bovine, Guinea pig, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.24 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human ENaC Alpha. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11176419
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sodium channel epithelial 1 alpha subunit , BESC2 , ENaCa , ENaCalpha , LIDLS3 , SCNEA , SCNN1
Cellular Localization
Apical cell membrane; Multi-pass membrane protein
Background
Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-ENaC Alpha antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110436](/templates/default/js/lightbox/images/loading.gif "Anti-ENaC Alpha antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110436")
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GTX110436 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SCNN1A(GTX110436) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110436 IHC-P Image
ENaC Alpha antibody [N3C2], Internal detects ENaC Alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ENaC Alpha stained by ENaC Alpha antibody [N3C2], Internal (GTX110436) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110436 ICC/IF Image
ENaC Alpha antibody [N3C2], Internal detects ENaC Alpha protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ENaC Alpha stained by ENaC Alpha antibody [N3C2], Internal (GTX110436) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX110436 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ENaC Alpha antibody [N3C2], Internal (GTX110436) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for ENaC Alpha antibody [N3C2], Internal (GTX110436). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ENaC Alpha antibody [N3C2], Internal (GTX110436). Be the first to share your experience with this product.
![Anti-ENaC Alpha antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110436](/upload/website/prouct_img/small/GTX110436/GTX110436_40863_IHC_w_23060500_877.webp "Anti-ENaC Alpha antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110436")
![Anti-ENaC Alpha antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110436](/upload/website/prouct_img/small/GTX110436/GTX110436_40863_20220617_IHC-P_M_22062121_206.webp "Anti-ENaC Alpha antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110436")
![Anti-ENaC Alpha antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110436](/upload/website/prouct_img/small/GTX110436/GTX110436_45070_20240621_ICC_IF_B_24070822_236.webp "Anti-ENaC Alpha antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110436")
![Anti-ENaC Alpha antibody [N3C2], Internal used in Western Blot (WB). GTX110436](/upload/website/prouct_img/small/GTX110436/GTX110436_45070_20231201_WB_M_tissue_23120519_110.webp "Anti-ENaC Alpha antibody [N3C2], Internal used in Western Blot (WB). GTX110436")
![Anti-ENaC Alpha antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110436](/upload/website/prouct_img/normal/GTX110436/GTX110436_40863_IHC_w_23060500_877.webp "Anti-ENaC Alpha antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110436")
![Anti-ENaC Alpha antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110436](/upload/website/prouct_img/normal/GTX110436/GTX110436_40863_20220617_IHC-P_M_22062121_206.webp "Anti-ENaC Alpha antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110436")
![Anti-ENaC Alpha antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110436](/upload/website/prouct_img/normal/GTX110436/GTX110436_45070_20240621_ICC_IF_B_24070822_236.webp "Anti-ENaC Alpha antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110436")
![Anti-ENaC Alpha antibody [N3C2], Internal used in Western Blot (WB). GTX110436](/upload/website/prouct_img/normal/GTX110436/GTX110436_45070_20231201_WB_M_tissue_23120519_110.webp "Anti-ENaC Alpha antibody [N3C2], Internal used in Western Blot (WB). GTX110436")