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ENaC Beta (phospho Thr615) antibody

Anti-ENaC Beta (phospho Thr615) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00671
Anti-ENaC Beta (phospho Thr615) antibody used in Western Blot (WB). GTX00671
Anti-ENaC Beta (phospho Thr615) antibody used in IHC (Paraffin sections) (IHC-P). GTX00671
Anti-ENaC Beta (phospho Thr615) antibody used in IHC (Paraffin sections) (IHC-P). GTX00671

Cat No. GTX00671

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($339)
See all ENaC Beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:500
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Nonvoltage-gated Sodium Channel 1 around the phosphorylation site of Thr615.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sodium channel epithelial 1 beta subunit , BESC1 , ENaCb , ENaCbeta , LIDLS1 , SCNEB

Cellular Localization

Apical cell membrane

Background

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the beta subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), and Liddle syndrome. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-ENaC Beta (phospho Thr615) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00671

GTX00671 ICC/IF Image

ICC/IF analysis of COS7 cells using GTX00671 ENaC Beta (phospho Thr615) antibody. The picture on the right is blocked with the phospho peptide.

Anti-ENaC Beta (phospho Thr615) antibody used in Western Blot (WB). GTX00671

GTX00671 WB Image

WB analysis of HeLa cell lysate using GTX00671 ENaC Beta (phospho Thr615) antibody. The lane on the right is blocked with the phospho peptide.

Anti-ENaC Beta (phospho Thr615) antibody used in IHC (Paraffin sections) (IHC-P). GTX00671

GTX00671 IHC-P Image

IHC-P analysis of human brain tissue using GTX00671 ENaC Beta (phospho Thr615) antibody. The picture on the right is blocked with the phospho peptide.

Anti-ENaC Beta (phospho Thr615) antibody used in IHC (Paraffin sections) (IHC-P). GTX00671

GTX00671 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX00671 ENaC Beta (phospho Thr615) antibody. Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339