GeneTex
United States (US)

EP300 antibody [3G230]

Cat No. GTX14984

Host Mouse
Clonality Monoclonal
Clone Name 3G230
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

For IF: Use at a concentration of 1 - 5 μg/ml. For IHC-P: Use at a concentration of 1-5 μg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For IP: Use at a concentration of 1μg/sample (Autoradiographic detection) For WB: Use at a concentration of 1 ug/ml. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

264 kDa. ( Note )

Positive Control

A431, HeLa or ML-1 cells

Specificity/Sensitivity

This product does not react with Mouse.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human p300 protein

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

E1A Binding Protein P300,Kat3B,Rsts2,P300,Ep300

Cellular Localization

Nuclear

Background

p300 is a transcriptional adapter protein (300 kDa) which is characterized by three cysteine and histidine rich regions and its C-terminus specifically binds the adenovirus E1A protein. p300 and p300 associated proteins are components of TATA-binding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated CBP (for CREB-binding protein). CBP and p300 are homologous to each other.

Database

Research Area

Package List Price ($)
$ 319