GeneTex
United States (US)

EP300 antibody [RW105]

GTX30618_1430_WB_18121410_942.jpg
GTX30618_101_FACS_18121410_225.jpg
GTX30618_100_FACS_18121410_771.jpg

Cat No. GTX30618

Host Mouse
Clonality Monoclonal
Clone Name RW105
Isotype IgG1
Application WB, ICC/IF, FACS, IP, ChIP assay, EM, in vitro
Reactivity Human, Mouse, Rat, Mustelid, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:250 - 1:500
ICC/IF 1:50 - 1:200
FACS Assay dependent
IP 1:10 - 1:500
ChIP assay Assay dependent
EM Assay dependent
in vitro Assay dependent
Not tested in other applications.

Calculated MW

264 kDa. ( Note )

Specificity/Sensitivity

This is specific for p300 protein. This recognizes residues 1921-2023.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

E1A Binding Protein P300,Kat3B,Rsts2,P300,Ep300

Cellular Localization

Nucleus

Background

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30618_1430_WB_18121410_942.jpg

GTX30618 WB Image

WB analysis of HeLa nuclear extract using GTX30618 EP300 antibody [RW105].
Dilution : 1:250

GTX30618_101_FACS_18121410_225.jpg

GTX30618 FACS Image

FACS (Intracellular staining) analysis of THP-1 cells using GTX30618 EP300 antibody [RW105].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

GTX30618_100_FACS_18121410_771.jpg

GTX30618 FACS Image

FACS (Intracellular staining) analysis of RAW264.7 cells using GTX30618 EP300 antibody [RW105].
Blue : Primary antibody
Orange : isotype control
Dilution : 2 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

Package List Price ($)
$ 319