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EP300 antibody [RW105]

Orthogonal
Anti-EP300 antibody [RW105] used in Flow cytometry (FACS). GTX30618
Anti-EP300 antibody [RW105] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30618
Anti-EP300 antibody [RW105] used in Flow cytometry (FACS). GTX30618
Anti-EP300 antibody [RW105] used in Western Blot (WB). GTX30618

Cat. No. GTX30618

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 RW105

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IP, ChIP assay, EM, in vitro

Reactivity

 Human, Mouse, Rat, Mustelid, Primate
Package
100 μg ($429)
See all EP300 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250 - 1:500
ICC/IF 1:50 - 1:200
FACS Assay dependent
IP 1:10 - 1:500
ChIP assay Assay dependent
EM Assay dependent
in vitro Assay dependent
Not tested in other applications.

Calculated MW

264 kDa. ( Note )

Product Note

This is specific for p300 protein. This recognizes residues 1921-2023.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E1A binding protein p300 , KAT3B , MKHK2 , RSTS2 , p300

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EP300 antibody [RW105] used in Flow cytometry (FACS). GTX30618

GTX30618 FACS Image

FACS (Intracellular staining) analysis of THP-1 cells using GTX30618 EP300 antibody [RW105].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-EP300 antibody [RW105] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30618

GTX30618 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX30618 EP300 antibody [RW105].
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.05% Triton X-100 in PBS
Dilution : 5 μg/mL

Anti-EP300 antibody [RW105] used in Flow cytometry (FACS). GTX30618

GTX30618 FACS Image

FACS (Intracellular staining) analysis of RAW264.7 cells using GTX30618 EP300 antibody [RW105].
Blue : Primary antibody
Orange : isotype control
Dilution : 2 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-EP300 antibody [RW105] used in Western Blot (WB). GTX30618

GTX30618 WB Image

WB analysis of HeLa nuclear extract using GTX30618 EP300 antibody [RW105].
Dilution : 1:250

REFERENCE

Application Reference

Su KW et al. Cells 2022; 11 (19) Thyroid Hormone Induces Oral Cancer Growth via the PD-L1-Dependent Signaling Pathway.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429