GeneTex
United States (US)

EP300 antibody [RW109]

GTX30619_1431_WB_18121410_530.jpg

Cat No. GTX30619

Host Mouse
Clonality Monoclonal
Clone Name RW109
Isotype IgG1
Application WB, ICC/IF, IP, ChIP assay
Reactivity Human, Mouse, Rat, Mustelid, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:100 - 1:200
IP 1:10 - 1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Specificity/Sensitivity

This is specific for p300 protein. This recognizes residues 2107-2283.
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.

Database

Research Area

DATA IMAGES
GTX30619_1431_WB_18121410_530.jpg

GTX30619 WB Image

WB analysis of HeLa nuclear extract using GTX30619 EP300 antibody [RW109].
Dilution : 1:1000

Package List Price ($)
$ 319