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EP300 antibody [RW128]

Anti-EP300 antibody [RW128] used in Western Blot (WB). GTX30614
Anti-EP300 antibody [RW128] used in Flow cytometry (FACS). GTX30614

Cat No. GTX30614

Host

Mouse

Clonality

Monoclonal

Clone Name

RW128

Isotype

IgG1

Application

WB, ICC/IF, FACS, IP

Reactivity

Human, Mouse, Rat, Mustelid, Primate
Package
200 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:250 - 1:500
ICC/IF 1:10 - 1:2000
FACS 1 μg/ml
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

264 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

E1A binding protein p300 , KAT3B , MKHK2 , RSTS2 , p300

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EP300 antibody [RW128] used in Western Blot (WB). GTX30614

GTX30614 WB Image

WB analysis of HeLa nuclear extract using GTX30614 EP300 antibody [RW128].
Dilution : 1:250

Anti-EP300 antibody [RW128] used in Flow cytometry (FACS). GTX30614

GTX30614 FACS Image

FACS (Intracellular staining) analysis of THP-1 cells using GTX30614 EP300 antibody [RW128].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

REFERENCE

There are currently no references for EP300 antibody [RW128] (GTX30614). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for EP300 antibody [RW128] (GTX30614). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339