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EP300 antibody

Anti-EP300 antibody used in IHC (Paraffin sections) (IHC-P). GTX134238
Anti-EP300 antibody used in IHC (Paraffin sections) (IHC-P). GTX134238
Anti-EP300 antibody used in Western Blot (WB). GTX134238

Cat No. GTX134238

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

264 kDa. ( Note )

Positive Control

A431 , A431 nuclear extract

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human EP300. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887243

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

E1A binding protein p300 , KAT3B , MKHK2 , RSTS2 , p300

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EP300 antibody used in IHC (Paraffin sections) (IHC-P). GTX134238

GTX134238 IHC-P Image

EP300 antibody detects EP300 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
EP300 stained by EP300 antibody (GTX134238) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EP300 antibody used in IHC (Paraffin sections) (IHC-P). GTX134238

GTX134238 IHC-P Image

EP300 antibody detects EP300 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EP300 stained by EP300 antibody (GTX134238) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EP300 antibody used in Western Blot (WB). GTX134238

GTX134238 WB Image

A431 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EP300 antibody (GTX134238) diluted at 1:500 and competitor's antibody (sc-584) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf