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EP300 antibody

Anti-EP300 antibody used in IHC (Paraffin sections) (IHC-P). GTX56157
Anti-EP300 antibody used in Western Blot (WB). GTX56157

Cat No. GTX56157

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

264 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of EP300. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887943

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

E1A binding protein p300 , KAT3B , MKHK2 , RSTS2 , p300

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EP300 antibody used in IHC (Paraffin sections) (IHC-P). GTX56157

GTX56157 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX56157 EP300 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-EP300 antibody used in Western Blot (WB). GTX56157

GTX56157 WB Image

WB analysis of A431 (A), HeLa (B), HT29 (C), A549 (D), NIH3T3 (E), PC12 (F) whole cell lysates using GTX56157 EP300 antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf