GeneTex
United States (US)

EPB41L3 antibody [C3], C-term

GTX101805_39577_IHC_19011506_622.jpg
GTX101805_40324_IFA_19011506_459.jpg
GTX101805_39577_WB_19011506_190.jpg

Cat No. GTX101805

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

Positive Control

PC-3 , U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EPB41L3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Erythrocyte Membrane Protein Band 4.1 Like 3,4.1B,Dal-1,Dal1,Epb41L3

Cellular Localization

Cytoplasm , cytoskeleton

Background

Critical growth regulator in the pathogenesis of meningiomas.

Database

Research Area

DATA IMAGES
GTX101805_39577_IHC_19011506_622.jpg

GTX101805 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using EPB41L3(GTX101805) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101805_40324_IFA_19011506_459.jpg

GTX101805 ICC/IF Image

EPB41L3 antibody [C3], C-term detects EPB41L3 protein at cytoplasm by confocal immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: EPB41L3 protein stained by EPB41L3 antibody [C3], C-term (GTX101805) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

GTX101805_39577_WB_19011506_190.jpg

GTX101805 WB Image

EPB41L3 antibody [C3], C-term detects EPB41L3 protein by western blot analysis.
A. 30 µg PC-3 whole cell lysate/extract
B. 30 µg U87-MG whole cell lysate/extract
5% SDS-PAGE
EPB41L3 antibody [C3], C-term (GTX101805) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf