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EPB41L3 antibody [C3], C-term

Anti-EPB41L3 antibody [C3], C-term used in Western Blot (WB). GTX101805
Anti-EPB41L3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101805
Anti-EPB41L3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101805
Anti-EPB41L3 antibody [C3], C-term used in Western Blot (WB). GTX101805

Cat. No. GTX101805

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EPB41L3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EPB41L3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240768

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erythrocyte membrane protein band 4.1 like 3 , 4.1B , DAL-1 , DAL1

Cellular Localization

Cytoplasm , cytoskeleton

Background

Critical growth regulator in the pathogenesis of meningiomas.

Database

Research Area

DATA IMAGES

Anti-EPB41L3 antibody [C3], C-term used in Western Blot (WB). GTX101805

GTX101805 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with EPB41L3 antibody [C3], C-term (GTX101805) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 24381168

Anti-EPB41L3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101805

GTX101805 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using EPB41L3(GTX101805) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EPB41L3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101805

GTX101805 ICC/IF Image

EPB41L3 antibody [C3], C-term detects EPB41L3 protein at cell membrane by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EPB41L3 stained by EPB41L3 antibody [C3], C-term (GTX101805) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-EPB41L3 antibody [C3], C-term used in Western Blot (WB). GTX101805

GTX101805 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EPB41L3 antibody [C3], C-term (GTX101805) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169