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EPHX1 antibody

Anti-EPHX1 antibody used in Western Blot (WB). GTX109360
Anti-EPHX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109360
Anti-EPHX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109360
Anti-EPHX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109360

Cat. No. GTX109360

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EPHX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950214

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epoxide hydrolase 1 , EPHX , EPOX , HYL1 , MEH

Cellular Localization

Microsome membrane; Single-pass membrane protein , Endoplasmic reticulum membrane; Single-pass membrane protein

Background

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Database

Research Area

DATA IMAGES

Anti-EPHX1 antibody used in Western Blot (WB). GTX109360

GTX109360 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
10% SDS PAGE
GTX109360 diluted at 1:1000

Anti-EPHX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109360

GTX109360 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1(GTX109360) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-EPHX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109360

GTX109360 IHC-P Image

EPHX1 antibody detects EPHX1 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
EPHX1 antibody (GTX109360) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EPHX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109360

GTX109360 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using Epoxide hydrolase 1(GTX109360) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production