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EPHX1 antibody

Anti-EPHX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110041
Anti-EPHX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110041
Anti-EPHX1 antibody used in Western Blot (WB). GTX110041

Cat. No. GTX110041

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EPHX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

H1299 , HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EPHX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950213

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epoxide hydrolase 1 , EPHX , EPOX , HYL1 , MEH

Cellular Localization

Microsome membrane; Single-pass membrane protein , Endoplasmic reticulum membrane; Single-pass membrane protein

Background

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Database

Research Area

DATA IMAGES

Anti-EPHX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110041

GTX110041 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft, using Epoxide hydrolase 1(GTX110041) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EPHX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110041

GTX110041 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1(GTX110041) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-EPHX1 antibody used in Western Blot (WB). GTX110041

GTX110041 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
10% SDS PAGE
GTX110041 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169