GeneTex
United States (US)

EPLIN antibody

Anti-EPLIN antibody used in Western Blot (WB). GTX31940
Anti-EPLIN antibody used in IHC (Paraffin sections) (IHC-P). GTX31940
Anti-EPLIN antibody used in IHC (Paraffin sections) (IHC-P). GTX31940

Cat No. GTX31940

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

Human Colon Tissue Lysate(GTX27928)

Specificity/Sensitivity

This antibody is human specific. At least four isoforms are known to exist; this antibody will only detect the three largest isoforms.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

EPLIN antibody was raised against an 18 amino acid peptide near the amino terminus of human EPLIN.The immunogen is located within amino acids 230 - 280 of EPLIN.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lim Domain And Actin Binding 1,Eplin,Ldlcq8,Srebp3,Lima1

Background

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
Anti-EPLIN antibody used in Western Blot (WB). GTX31940

GTX31940 WB Image

WB analysis of human colon tissue lysate using GTX31940 EPLIN antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-EPLIN antibody used in IHC (Paraffin sections) (IHC-P). GTX31940

GTX31940 IHC-P Image

IHC-P analysis of human colon tissue using GTX31940 EPLIN antibody.
Working concentration : 20 μg/ml

Anti-EPLIN antibody used in IHC (Paraffin sections) (IHC-P). GTX31940

GTX31940 IHC-P Image

IHC-P analysis of human colon tissue using GTX31940 EPLIN antibody.
Working concentration : 5 μg/ml