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EPO antibody [85]

Anti-EPO antibody [85] used in Western Blot (WB). GTX19485

Cat. No. GTX19485

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 85

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human
Package
250 μg ($399)
See all EPO products

APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

21 kDa. ( Note )

Product Note

Does not cross react with other human cytokines tested such as IL-8, IL-16, IL-7, MCP-1, TNF-beta, GM-CSF, M-CSF, IL-1 beta, bFGF, and human serum albumin or bovine serum albumin

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified CHO cell-expressed recombinant human EPO.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erythropoietin , DBAL , ECYT5 , EP , MVCD2

Cellular Localization

Secreted

Background

This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-EPO antibody [85] used in Western Blot (WB). GTX19485

GTX19485 WB Image

Western blot analysis detecting EPO antigen (100 ng/well) using mouse anti-human EPO antibody (clone 85) at 1:1000 dilution (1 ug/mL)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations