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EPO antibody [HL1794]

Recombinant antibody Orthogonal
Anti-EPO antibody [HL1794] used in Western Blot (WB). GTX637446
Anti-EPO antibody [HL1794] used in ELISA (ELISA). GTX637446
Anti-EPO antibody [HL1794] used in ELISA (ELISA). GTX637446
Anti-EPO antibody [HL1794] used in IHC (Paraffin sections) (IHC-P). GTX637446
Anti-EPO antibody [HL1794] used in IHC (Paraffin sections) (IHC-P). GTX637446
Anti-EPO antibody [HL1794] used in ELISA (ELISA). GTX637446
Anti-EPO antibody [HL1794] used in ELISA (ELISA). GTX637446
Anti-EPO antibody [HL1794] used in Western Blot (WB). GTX637446
Anti-EPO antibody [HL1794] used in Western Blot (WB). GTX637446

Cat. No. GTX637446

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1794

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all EPO products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX637446/GTX637445, Detection : GTX637445/GTX637446.
Please notice that GTX637445/GTX637446 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637446/GTX637445, Please contact us for custom HRP-conjugated antibody.
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human EPO

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erythropoietin , DBAL , ECYT5 , EP , MVCD2

Cellular Localization

Secreted

Background

This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-EPO antibody [HL1794] used in Western Blot (WB). GTX637446

GTX637446 WB Image

Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-EPO antibody [HL1794] used in ELISA (ELISA). GTX637446

GTX637446 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (GTX00422-pro) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (GTX637446) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-EPO antibody [HL1794] used in ELISA (ELISA). GTX637446

GTX637446 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (GTX637446) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-EPO antibody [HL1794] used in IHC (Paraffin sections) (IHC-P). GTX637446

GTX637446 IHC-P Image

EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
EPO stained by EPO antibody [HL1794] (GTX637446) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EPO antibody [HL1794] used in IHC (Paraffin sections) (IHC-P). GTX637446

GTX637446 IHC-P Image

EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
EPO stained by EPO antibody [HL1794] (GTX637446) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EPO antibody [HL1794] used in ELISA (ELISA). GTX637446

GTX637446 ELISA Image

Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below.

Capture: EPO antibody [HL1794] (GTX637446) (5 μg/mL)
Detection: HRP-conjugated EPO antibody [HL1793] (GTX637445) (1 μg/mL)
Please notice that GTX637445 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637446. Please contact us for custom HRP-conjugated antibody.

Anti-EPO antibody [HL1794] used in ELISA (ELISA). GTX637446

GTX637446 ELISA Image

Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below.

Capture: EPO antibody [HL1793] (GTX637445) (5 μg/mL)
Detection: HRP-conjugated EPO antibody [HL1794] (GTX637446) (1 μg/mL)
Please notice that GTX637446 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637445. Please contact us for custom HRP-conjugated antibody.

Anti-EPO antibody [HL1794] used in Western Blot (WB). GTX637446

GTX637446 WB Image

Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EPO antibody [HL1794] used in Western Blot (WB). GTX637446

GTX637446 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations