Sandwich ELISA detection of recombinant (CHO cells expressed, full-length human EPO protein) using antibodies as below.

Capture: EPO antibody [HL1793] (GTX637445) (5 μg/mL)
Detection: HRP-conjugated EPO antibody [HL1794]  (GTX637446-01) (1 μg/mL

The cell proliferation of the TF-1 human erythroleukemic cell line induced by Human Erythropoietin/EPO and neutralization by Erythropoietin Antibody. Proliferation elicited by Recombinant Human Erythropoietin/EPO (0.5 ng/mL) is neutralized by increasing concentrations of EPO antibody [HL1794] (GTX637446).

EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
EPO stained by EPO antibody [HL1794] (GTX637446) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
EPO stained by EPO antibody [HL1794] (GTX637446) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below.

Capture: EPO antibody [HL1793] (GTX637445) (5 μg/mL)
Detection: HRP-conjugated EPO antibody [HL1794] (GTX637446) (1 μg/mL)
Please notice that GTX637446 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637445. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below.

Capture: EPO antibody [HL1794] (GTX637446) (5 μg/mL)
Detection: HRP-conjugated EPO antibody [HL1793] (GTX637445) (1 μg/mL)
Please notice that GTX637445 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637446. Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (GTX637446) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (GTX00422-pro) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (GTX637446) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (GTX637446) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.