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EPO antibody [rEPO/1367]

Anti-EPO antibody [rEPO/1367] used in IHC (Paraffin sections) (IHC-P). GTX02632

Cat No. GTX02632

Host

Mouse

Clonality

Monoclonal

Clone Name

rEPO/1367

Isotype

IgG1

Application

IHC-P, ELISA

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 2-4 μg/ml
ELISA 2-4 μg/ml (for coating)

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 28-162) of human EPO protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

erythropoietin , DBAL , ECYT5 , EP , MVCD2

Cellular Localization

Secreted

Background

This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-EPO antibody [rEPO/1367] used in IHC (Paraffin sections) (IHC-P). GTX02632

GTX02632 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX02632 EPO antibody [rEPO/1367].

REFERENCE

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REVIEW

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