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EPO antibody

Anti-EPO antibody used in Western Blot (WB). GTX100813
Anti-EPO antibody used in IHC (Paraffin sections) (IHC-P). GTX100813
Anti-EPO antibody used in ELISA (ELISA). GTX100813
Anti-EPO antibody used in Western Blot (WB). GTX100813
Anti-EPO antibody used in ELISA (ELISA). GTX100813

Cat. No. GTX100813

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EPO products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

rPT EPO

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EPO. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619411

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erythropoietin , DBAL , ECYT5 , EP , MVCD2

Cellular Localization

Secreted

Background

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EPO antibody used in Western Blot (WB). GTX100813

GTX100813 WB Image

Recombinant human EPO protein (with glycosylation) expressed in CHO cells (100 ng) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody (GTX100813) diluted at 1:10000.

Anti-EPO antibody used in IHC (Paraffin sections) (IHC-P). GTX100813

GTX100813 IHC-P Image

EPO antibody detects EPO protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
EPO antibody (GTX100813) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EPO antibody used in ELISA (ELISA). GTX100813

GTX100813 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (GTX00422-pro) (500-7.81 ng/mL). Coated protein was probed with EPO antibody (GTX100813) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-EPO antibody used in Western Blot (WB). GTX100813

GTX100813 WB Image

Recombinant mouse EPO protein (100 ng) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody (GTX100813) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EPO antibody used in ELISA (ELISA). GTX100813

GTX100813 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (23.81-0.37 nM). Coated protein was probed with EPO antibody (GTX100813) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169