GeneTex
United States (US)

EPO antibody

GTX100813_40891_20160727_IHC-P_R_19011506_788.jpg
GTX100813_40891_20170126_WB_rProtein_19011506_867.jpg
GTX100813_40891_20170817_ELISA_19011506_104.jpg

Cat No. GTX100813

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

rPT EPO

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EPO. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Erythropoietin,Dbal,Ecyt5,Ep,Mvcd2,Epo

Cellular Localization

Secreted

Background

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100813_40891_20160727_IHC-P_R_19011506_788.jpg

GTX100813 IHC-P Image

EPO antibody detects EPO protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
EPO antibody (GTX100813) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100813_40891_20170126_WB_rProtein_19011506_867.jpg

GTX100813 WB Image

Recombinant human EPO protein (with glycosylation) expressed in CHO cells (100 ng) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody (GTX100813) diluted at 1:10000.

GTX100813_40891_20170817_ELISA_19011506_104.jpg

GTX100813 ELISA Image

An ELISA plate is coated with 50 µL of EPO recombinant protein at concentration ranged from 0.125 µg/mL to 4 µg/mL. The coated protein is detected with EPO antibody (GTX100813) at 1 µg/mL.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf