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EPO antibody

Anti-EPO antibody used in Western Blot (WB). GTX112833
Anti-EPO antibody used in ELISA (ELISA). GTX112833

Cat. No. GTX112833

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Recombinant human EPO protein

Predict Reactivity

Mouse, Rat, Rabbit, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human EPO Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176454

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erythropoietin , DBAL , ECYT5 , EP , MVCD2

Cellular Localization

Secreted

Background

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EPO antibody used in Western Blot (WB). GTX112833

GTX112833 WB Image

Recombinant human EPO protein (with glycosylation) expressed in CHO cells (20 and 100 ng) were separated by 12% SDS-PAGE, and the membrane was blotted with (GTX112833) diluted at 1:20000.

Anti-EPO antibody used in ELISA (ELISA). GTX112833

GTX112833 ELISA Image

An ELISA plate is coated with 50 μL of EPO recombinant protein at concentration ranged from 0.125 μg/mL to 4 μg/mL. The coated protein is detected with EPO antibody (GTX112833) at 1 μg/mL.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169