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EPRS antibody

Anti-EPRS antibody used in IHC (Paraffin sections) (IHC-P). GTX130848
Anti-EPRS antibody used in Immunoprecipitation (IP). GTX130848
Anti-EPRS antibody used in IHC (Paraffin sections) (IHC-P). GTX130848
Anti-EPRS antibody used in Western Blot (WB). GTX130848

Cat. No. GTX130848

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm, IP

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all EPRS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

171 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EPRS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886363

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamyl-prolyl-tRNA synthetase , EARS , GLUPRORS , HLD15 , PARS , PIG32 , QARS , QPRS

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EPRS antibody used in IHC (Paraffin sections) (IHC-P). GTX130848

GTX130848 IHC-P Image

EPRS antibody detects EPRS protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EPRS antibody (GTX130848) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EPRS antibody used in Immunoprecipitation (IP). GTX130848

GTX130848 IP Image

Immunoprecipitation of EPRS protein from 293T whole cell extracts using 5 μg of EPRS antibody (GTX130848).
Western blot analysis was performed using EPRS antibody (GTX130848) diluted at 1:2500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-EPRS antibody used in IHC (Paraffin sections) (IHC-P). GTX130848

GTX130848 IHC-P Image

EPRS antibody detects EPRS protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
EPRS antibody (GTX130848) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EPRS antibody used in Western Blot (WB). GTX130848

GTX130848 WB Image

EPRS antibody detects EPRS protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with EPRS antibody (GTX130848) diluted at a dilution of 1:1000.

REFERENCE

Application Reference

Lu X et al. EMBO Rep 2020; 21 (12) : e49684 The tRNA-like small noncoding RNA mascRNA promotes global protein translation.
Submit a Reference

REVIEW

EPRS antibody Cat. No. GTX130848

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 1 users reviews)
Anti-EPRS antibody used in IHC (Whole mount) (IHC-Wm). GTX130848
Date : Anonymous submitted on 27-Aug-2018
Score :
Application Tested : IHC-Wm
Sample Species : Dm
Sample : Drosophila eye
Fixation : 4% PFA
Primary Antibody : 1:50, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169