Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
EPS8 antibody
GTX100254 diluted at 1:1000

Immunoprecipitation of EPS8 protein from HepG2 whole cell extracts using 5 μg of EPS8 antibody [C3], C-term (GTX100254).
Western blot analysis was performed using EPS8 antibody [C3], C-term (GTX100254).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EPS8 antibody [C3], C-term (GTX100254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EPS8 antibody [C3], C-term detects EPS8 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EPS8 antibody [C3], C-term (GTX100254) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min