GeneTex
United States (US)

EPS8 antibody [C3], C-term

GTX100254_WB_19011506_195.jpg
GTX100254_39568_20150529_IP_19011506_589.jpg
GTX100254_41724_IHC_19011506_686.jpg
GTX100254_39568_20170518_WB_M_R_19011506_388.jpg

Cat No. GTX100254

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EPS8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EPS8 antibody, epidermal growth factor receptor kinase substrate 8 antibody, epidermal growth factor receptor pathway substrate 8 antibody

Background

This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100254_WB_19011506_195.jpg

GTX100254 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
EPS8 antibody
GTX100254 diluted at 1:1000

GTX100254_39568_20150529_IP_19011506_589.jpg

GTX100254 IP Image

Immunoprecipitation of EPS8 protein from HepG2 whole cell extracts using 5 μg of EPS8 antibody [C3], C-term (GTX100254).
Western blot analysis was performed using EPS8 antibody [C3], C-term (GTX100254).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100254_41724_IHC_19011506_686.jpg

GTX100254 IHC-P Image

EPS8 antibody [C3], C-term detects EPS8 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EPS8 antibody [C3], C-term (GTX100254) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100254_39568_20170518_WB_M_R_19011506_388.jpg

GTX100254 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EPS8 antibody [C3], C-term (GTX100254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169