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EPS8 antibody [C3], C-term

Anti-EPS8 antibody [C3], C-term used in Western Blot (WB). GTX100254
Anti-EPS8 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100254
Anti-EPS8 antibody [C3], C-term used in Western Blot (WB). GTX100254
Anti-EPS8 antibody [C3], C-term used in Immunoprecipitation (IP). GTX100254

Cat. No. GTX100254

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EPS8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

H1299 , HeLa , mouse brain , rat brain

Predict Reactivity

Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EPS8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240772

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor receptor pathway substrate 8 , DFNB102

Background

This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EPS8 antibody [C3], C-term used in Western Blot (WB). GTX100254

GTX100254 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
EPS8 antibody
GTX100254 diluted at 1:1000

Anti-EPS8 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100254

GTX100254 IHC-P Image

EPS8 antibody [C3], C-term detects EPS8 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EPS8 antibody [C3], C-term (GTX100254) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EPS8 antibody [C3], C-term used in Western Blot (WB). GTX100254

GTX100254 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EPS8 antibody [C3], C-term (GTX100254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EPS8 antibody [C3], C-term used in Immunoprecipitation (IP). GTX100254

GTX100254 IP Image

Immunoprecipitation of EPS8 protein from HepG2 whole cell extracts using 5 μg of EPS8 antibody [C3], C-term (GTX100254).
Western blot analysis was performed using EPS8 antibody [C3], C-term (GTX100254).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169