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EPXH2 antibody [2F2]

Anti-EPXH2 antibody [2F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84567
Anti-EPXH2 antibody [2F2] used in Flow cytometry (FACS). GTX84567
Anti-EPXH2 antibody [2F2] used in Western Blot (WB). GTX84567

Cat. No. GTX84567

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2F2

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($519)
See all EPXH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human EPHX2 (NP_001970) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10731826

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epoxide hydrolase 2 , ABHD20 , CEH , SEH

Cellular Localization

Cytoplasm

Background

Acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Has low phosphatase activity.

Database

Research Area

DATA IMAGES

Anti-EPXH2 antibody [2F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84567

GTX84567 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with EPXH2 plasmid using GTX84567 EPXH2 antibody [2F2].

Anti-EPXH2 antibody [2F2] used in Flow cytometry (FACS). GTX84567

GTX84567 FACS Image

FACS analysis of HEK293T cells transfected with either EPXH2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84567 EPXH2 antibody [2F2].

Anti-EPXH2 antibody [2F2] used in Western Blot (WB). GTX84567

GTX84567 WB Image

WB analysis of HEK293T cells transfected with EPXH2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84567 EPXH2 antibody [2F2].
Loading : 5 ug per lane

REFERENCE

Application Reference

Pistorius K et al. Cell Chem Biol 2018; 25 (6) : 749-760.e9 PDn-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519