ER81 antibody

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data are based on NCBI database.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ER81 antibody detects ER81 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ER81 stained by ER81 antibody (GTX129202) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ER81 antibody detects ER81 protein by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ER81 stained by ER81 antibody (GTX129202) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

ER81 antibody detects ER81 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ER81 stained by ER81 antibody (GTX129202) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ER81 antibody detects ER81 protein at nucleus by immunofluorescent analysis.
Sample: C8D30 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ER81 protein stained by ER81 antibody (GTX129202) diluted at 1:500.
Blue: Hoechst 33342 staining.

ER81 antibody detects ER81 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: ER81 stained by ER81 antibody (GTX129202) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

ER81 antibody detects ER81 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ER81 stained by ER81 antibody (GTX129202) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ER81 antibody detects ER81 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ER81 stained by ER81 antibody (GTX129202) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ER81 antibody detects ER81 protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ER81 stained by ER81 antibody (GTX129202) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.