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ER81 antibody

Anti-ER81 antibody used in Western Blot (WB). GTX129202
Anti-ER81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129202
Anti-ER81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129202
Anti-ER81 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129202
Anti-ER81 antibody used in Western Blot (WB). GTX129202
Anti-ER81 antibody used in Western Blot (WB). GTX129202

Cat No. GTX129202

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A431 , Rat2

Specificity/Sensitivity

This antibody was rasied against ER81 isoform 1. Based on sequence homology, this antibody can recognize most ER81 isoforms, but it may be unable to recognize isoform 4 according to the customer's feedback.

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ER81. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ETS variant 1 , ER81

Cellular Localization

Nucleus

Background

This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-ER81 antibody used in Western Blot (WB). GTX129202

GTX129202 WB Image

U87-MG whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 23774214

Anti-ER81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129202

GTX129202 ICC/IF Image

ER81 antibody detects ER81 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ER81 stained by ER81 antibody (GTX129202) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ER81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129202

GTX129202 ICC/IF Image

ER81 antibody detects ER81 protein at nucleus by immunofluorescent analysis.
Sample: C8D30 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ER81 protein stained by ER81 antibody (GTX129202) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ER81 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129202

GTX129202 IHC-Fr Image

ER81 antibody detects ER81 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: ER81 stained by ER81 antibody (GTX129202) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ER81 antibody used in Western Blot (WB). GTX129202

GTX129202 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ER81 antibody used in Western Blot (WB). GTX129202

GTX129202 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ER81 antibody (GTX129202) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169
Currently in production