APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HepG2
Predict Reactivity
Mouse, Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ERAB. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036853
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hydroxysteroid 17-beta dehydrogenase 10 , 17b-HSD10 , ABAD , CAMR , DUPXp11.22 , ERAB , HADH2 , HCD2 , HSD10MD , MHBD , MRPP2 , MRX17 , MRX31 , MRXS10 , SCHAD , SDR5C1
Cellular Localization
Mitochondrion
Background
This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-ERAB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113455](/templates/default/js/lightbox/images/loading.gif "Anti-ERAB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113455")
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GTX113455 IHC-P Image
ERAB antibody [N2C3] detects ERAB protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ERAB antibody [N2C3] (GTX113455) dilution: 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113455 WB Image
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX113455 diluted at 1:1000
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REFERENCE
There are currently no references for ERAB antibody [N2C3] (GTX113455). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ERAB antibody [N2C3] (GTX113455). Be the first to share your experience with this product.
![Anti-ERAB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113455](/upload/website/prouct_img/small/GTX113455/GTX113455_40135_IHC_w_23051501_162.webp "Anti-ERAB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113455")
![Anti-ERAB antibody [N2C3] used in Western Blot (WB). GTX113455](/upload/website/prouct_img/small/GTX113455/GTX113455_WB_w_23051501_960.webp "Anti-ERAB antibody [N2C3] used in Western Blot (WB). GTX113455")
![Anti-ERAB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113455](/upload/website/prouct_img/normal/GTX113455/GTX113455_40135_IHC_w_23051501_162.webp "Anti-ERAB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113455")
![Anti-ERAB antibody [N2C3] used in Western Blot (WB). GTX113455](/upload/website/prouct_img/normal/GTX113455/GTX113455_WB_w_23051501_960.webp "Anti-ERAB antibody [N2C3] used in Western Blot (WB). GTX113455")