GeneTex
United States (US)

ERAB antibody

GTX100301_39428_20170712_IFA_19011506_953.jpg
GTX100301_39428_WB_M_19011506_821.jpg
GTX100301_39428_IHC_M_3_19011506_734.jpg
GTX100301_39428_IHC_M_19011506_528.jpg
GTX100301_WB_19011506_386.jpg
GTX100301_39428_WB_R_19011506_740.jpg
GTX100301_39428_IHC_19011506_232.jpg
GTX100301_39428_IHC_M_2_19011506_985.jpg

Cat No. GTX100301

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HeLaS3 , Molt-4 , Neuro2A , GL261 , PC-12

Predict Reactivity

Zebrafish, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERAB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hydroxysteroid 17-Beta Dehydrogenase 10,17B-Hsd10,Abad,Camr,Dupxp11.22,Erab,Hadh2,Hcd2,Hsd10Md,Mhbd,Mrpp2,Mrx17,Mrx31,Mrxs10,Schad,Sdr5C1,Hsd17B10

Cellular Localization

Mitochondrion

Background

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100301_39428_20170712_IFA_19011506_953.jpg

GTX100301 ICC/IF Image

ERAB antibody detects ERAB protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ERAB protein stained by ERAB antibody (GTX100301) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100301_39428_WB_M_19011506_821.jpg

GTX100301 WB Image

Sample (30 ug of whole cell lysate)
  A: Neuro 2A
B: GL261
12% SDS PAGE 
GTX100301 diluted at 1:1000

GTX100301_39428_IHC_M_3_19011506_734.jpg

GTX100301 IHC-P Image

ERAB antibody detects ERAB protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ERAB antibody (GTX100301) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100301_39428_IHC_M_19011506_528.jpg

GTX100301 IHC-P Image

ERAB antibody detects ERAB protein at mitochondria on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
ERAB antibody (GTX100301) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100301_WB_19011506_386.jpg

GTX100301 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
B:MOLT4 (GTX27912)
15% SDS PAGE
GTX100301 diluted at 1:1000

GTX100301_39428_WB_R_19011506_740.jpg

GTX100301 WB Image

Sample (30 ug of whole cell lysate) 
  A: PC-12
  12% SDS PAGE 
  GTX100301 diluted at 1:5000

GTX100301_39428_IHC_19011506_232.jpg

GTX100301 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ERAB(GTX100301) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100301_39428_IHC_M_2_19011506_985.jpg

GTX100301 IHC-P Image

ERAB antibody detects ERAB protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ERAB antibody (GTX100301) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169