APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-2μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX48613-PEP).
Product Note
This antibody is expected to recognise both reported isoforms.
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CIRLDGAIRMQP, from the C Terminus of the protein sequence according to NP_004484.1; NP_001032900.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
RRID
AB_10617650
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hydroxysteroid 17-beta dehydrogenase 10 , 17b-HSD10 , ABAD , CAMR , DUPXp11.22 , ERAB , HADH2 , HCD2 , HSD10MD , MHBD , MRPP2 , MRX17 , MRX31 , MRXS10 , SCHAD , SDR5C1
Cellular Localization
Mitochondrion
Background
This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Aug 2014]
Database
Research Area
DATA IMAGES
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GTX48613 WB Image
WB analysis of human brain lysate using GTX48613 ERAB antibody.
Dilution : 0.3μg/ml
Loading : 30μg protein in RIPA buffer
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GTX48613 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 26960901
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REFERENCE
REVIEW
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