GeneTex
United States (US)

ERCC1 antibody, N-term

GTX88627_2055_WB_18121411_724.jpg

Cat No. GTX88627

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88627-PEP).

Specificity/Sensitivity

This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence DPGKDKEGVPQPS-C, from the N Terminus of the protein sequence according to NP_973730.1; NP_001974.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ercc Excision Repair 1, Endonuclease Non-Catalytic Subunit,Cofs4,Rad10,Uv20,Ercc1

Background

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES
GTX88627_2055_WB_18121411_724.jpg

GTX88627 WB Image

WB analysis of A431 lysate using GTX88627 ERCC1 antibody, N-term.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329