Wild-type (WT) and ERCC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERCC1 antibody (GTX129282) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ERCC1 antibody detects ERCC1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
ERCC1 antibody (GTX129282) dilution: 1:1000

ERCC1 antibody detects ERCC1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERCC1 protein stained by ERCC1 antibody (GTX129282) diluted at 1:1000.
Blue: Hoechst 33342 staining.

ERCC1 antibody detects ERCC1 protein at cytoplasm and nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
ERCC1 antibody (GTX129282) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ERCC1 antibody detects ERCC1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
ERCC1 antibody (GTX129282) dilution: 1:1000