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ERCC1 antibody

Anti-ERCC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33185
Anti-ERCC1 antibody used in Western Blot (WB). GTX33185
Anti-ERCC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33185

Cat No. GTX33185

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human ERCC1 (NP_973730.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ERCC excision repair 1, endonuclease non-catalytic subunit , COFS4 , RAD10 , UV20

Cellular Localization

Nucleus

Background

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-ERCC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33185

GTX33185 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33185 ERCC1 antibody.
Blue : DAPI

Anti-ERCC1 antibody used in Western Blot (WB). GTX33185

GTX33185 WB Image

WB analysis of various sample lysates using GTX33185 ERCC1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-ERCC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33185

GTX33185 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33185 ERCC1 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329